The Application Layer is the layer closest to the end user. When you are using a program that is going to send something or contact someone over the internet you are using a network application that operates at the Application Layer. Each program/application that sends data over a network is identified by a particular protocol, at Layer 4 this protocol is associated with a port number. For example a web browser like Internet Explorer requests and receives pages from web servers its protocol is HTTP the hyper text transfer protocol and its correlative port number is port 80.
This is a diagram of the OSI and TCP/IP Models and how they correspond to PDUs, Protocols and Devices
This is a visual diagram of the process data goes through when sent over a network in a layered architecture
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